2.5 Enzymes
IB Syllabus Statements
Understandings:
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Enzymes have an active site to which specific substrates bind.
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Enzyme catalysis involves molecular motion and the collision of substrates with the active site.
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Temperature, pH and substrate concentration affect the rate of activity of enzymes.
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Enzymes can be denatured.
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Immobilized enzymes are widely used in industry.
Applications and skills:
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Application: Methods of production of lactose-free milk and its advantages.
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Skill: Design of experiments to test the effect of temperature, pH and substrate concentration on the activity of enzymes.
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Skill: Experimental investigation of a factor affecting enzyme activity. (Practical 3)
Videos
Enzymes Introduction
Enzymes in Technology
Factors that affect Enzyme Activity
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Topic 2.5 Enzymes SSaQ (Syllabus Statement as Questions)
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Outline the role of the active site in enzymes.
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Explain how enzymes function including the role of molecular motion and collision.
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Outline how temperature, pH, and substrate concentration affect the rate of enzyme activity. Use graphs to illustrate your answer.
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Explain denaturation.
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Explain how immobilized enzymes are used in the production of lactose-free milk.
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What are the advantages of producing lactose-free milk?
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Outline how you would set up an experiment to test the effect of temperature, pH, or substrate concentration on the activity of enzymes.
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Explain the importance of performing replicates in experiments.